Regulatory

Part:BBa_K187003:Design

Designed by: Team BioBytes   Group: iGEM09_Alberta   (2009-10-16)
Revision as of 02:28, 17 October 2009 by JuliaPon (Talk | contribs) (→‎Design Notes)

Sigma 70 1% promoter in pAB BioBytes plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
    Illegal PstI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 47
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
    Illegal PstI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
    Illegal PstI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The 1% promoter produces ~1% of the expression level from a consensus promoter. The pAB plasmid adapts the promoter for assembly with other parts using the BioBytes assembly system. See the BioBytes RFC for details on this method.

Source

1% promoter was modified from Anderson collection of promoters, part BBa_J23113 pAB was derived from pUC19

References