Coding

Part:BBa_K4606001:Design

Designed by: Baochao Li   Group: iGEM23_Nanjing-NFLS   (2023-09-14)
Revision as of 01:51, 23 September 2023 by Alex2006 (Talk | contribs) (→‎References)

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ZsGreen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added ZsGreen to the transfection plasmid. In this way, when the plasmid was successfully transfected into the recipient cells, the ZsGreen was expressed, and the green fluorescence could be observed under the microscope. We can observe the success rate of plasmid transfection by this protein.

Source

The fluorescent protein ZsGreen was selected from a combinatorial protein display library.

References

M'baye M, Hua G, Khan HA, Yang L. RNAi-mediated knockdown of INHBB increases apoptosis and inhibits steroidogenesis in mouse granulosa cells. J Reprod Dev. 2015;61(5):391-397. doi:10.1262/jrd.2014-158