Composite

Part:BBa_K4711026

Designed by: Qingyu Wu   Group: iGEM23_OUC-China   (2023-09-22)
Revision as of 09:03, 10 October 2023 by Wuqingyu (Talk | contribs) (→‎Usage and Biology)


T7+alkB+alkG+T7+alkT+Spycatcher

Usage and Biology

Colony PCR results showed the appearance of the target band at around 4000 bp, indicating successful plasmid integration into Escherichia coli. SDS-PAGE analysis revealed the successful expression of the AlkB, AlkG, and AlkT proteins. Additionally, by setting a gradient of IPTG concentrations, it was observed that the protein expression was most efficient when induced with 0.5 mM IPTG, yielding the highest protein expression levels.

Fig 1 (A) Gene circuit (B) Colony PCR (C) SDS-PAGE M: Protein Marker 1: 0mM IPTG 2: 0.2mM IPTG 3: 0.5mM IPTG 4: 1mM IPTG. The protein bands from top to bottom are alkT (56KDa), alkB (45KDa), and alkG (18KDa).

Source

Potential applications

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1860
    Illegal NheI site found at 3534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1634
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 167
    Illegal NgoMIV site found at 1079
    Illegal NgoMIV site found at 2554
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1966


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