Part:BBa_K4711019

OleTJE P450

Usage and Biology

We utilize the OleTJE P450 enzyme to catalyze the single-step decarboxylation of fatty acids to produce α-olefins, which is a relatively simple approach in the biosynthesis of α-olefins. It directly uses free fatty acids as substrates to synthesize α-olefins in one step. This process is simple and easy to control, making it highly regarded for its potential in designing biological systems for α-olefin production. The degradation mechanism is shown in the diagram below:

Fig 1 P450 catalytic process and synthesis of α-olefin

Fig 2 The 3D structure of GDH

Source

Jeotgalicoccus sp. 8456 bacterium

References

[1]Belcher J, McLean KJ, Matthews S, Woodward LS, Fisher K, Rigby SEJ, Nelson DR, Potts D, Baynham MT, Parker DA, Leys D, Munro AW. Structure and biochemical properties of the alkene producing cytochrome P450 OleTJE (CYP152L1) from the Jeotgalicoccus sp. 8456 bacterium. J Biol Chem. 2014 Mar 7;289(10):6535-6550. doi: 10.1074/jbc.M113.527325. Epub 2014 Jan 18. PMID: 24443585; PMCID: PMC3945318.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1003
  Illegal PstI site found at 932
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1003
  Illegal PstI site found at 932
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1003
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1003
  Illegal PstI site found at 932
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1003
  Illegal PstI site found at 932
  
• 1000
  COMPATIBLE WITH RFC[1000]