Part:BBa_K283003

pTet-rbs-chez_histag-double terminator

This part was designed to generate the cheZ proteins which are responsible for the dephosphorylation of cheY protein involved in bacteria flagella movement. A 6-histidine tail is added to the cheZ encoding region. The pTet can be repressed by the TetR repressor protein(C0040). It is constitutively on without the presence of TetR repressor. The backbone of this biobrick is based on BBa_J61003. CheZ_his tag and strong double terminators lie between XbaI and SpeI.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 726
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]