Part:BBa_K283036
invasin(1-625aa)
The invasin protein of Yersinia pseudotuberculosis has been utilized for heterologous surface display. Y. pseudotuberculosis invasin promotes bacterial entry by binding to host cell integrins. It is a 986-residue protein of which the 500 N-terminal aa are thought to reside in the outer membrane. The C-terminal 497 residues, which make up the extracellular part, are involved in the binding to integrin. Nakajima and co-workers developed a system for expression of random peptides on the cell surface of E. coli by creation of a fusion hybrid between a peptide and the invasin protein. The fusion protein consists of the first 625 aa of invasin, a six aa long proline spacer, and a decamer of random peptides flanked by cysteine residues.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 886
- 1000COMPATIBLE WITH RFC[1000]
//proteindomain/localization
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