Part:BBa_K4586009

lamp2b

Part Description

This part is the gene coding for a member of a family of membrane glycoproteins called Lysosome-associated membrane protein 2 (LAMP2), through modification of the outer membrane domain of the lamp2b. Our target protein can be delivered to the exosome's surface using Lamp 2b.

Usage

This part is used to express citrullinated vimentin on the exosomal surface to increase the exosomes' affinity for the autoreactive B-cells, as this transmembrane protein is unique to the membrane of exosomes and lysosomes, so the likelihood of exosome and B-cell fusion has significantly increased as shown in figure 1.

Figure 1: This figure illustrates the construction of our engineered exosomes that express citrullinated vimentin on their membranes conjugated to a transmembrane protein known as lysosome-associated membrane glycoprotein 2b (lamp2b), which is unique to the membrane of exosomes.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]