DNA

Part:BBa_K4890001

Designed by: Duoqing LIN   Group: iGEM23_Shanghai-SDG   (2023-08-14)
Revision as of 10:19, 5 October 2023 by Htclamm (Talk | contribs)

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MTF1

This part is MTF-1 gene derived from Drosophila melanogaster. It encodes MTF-1 (metal-responsive transcription factor-1) which is a transcription factor. MTF-1 protein can be activated by heavy metals. In the cell nucleus of Drosophila cells, MTF-1 binds to MRE to recruit RNA polymerase, in turn increasing the expression of Mto/Mtn to detoxicate metals.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 1923
    Illegal PstI site found at 1681
    Illegal PstI site found at 1859
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 1923
    Illegal NheI site found at 136
    Illegal PstI site found at 1681
    Illegal PstI site found at 1859
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 1923
    Illegal BglII site found at 154
    Illegal BamHI site found at 106
    Illegal BamHI site found at 1095
    Illegal BamHI site found at 1491
    Illegal BamHI site found at 1518
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 1923
    Illegal PstI site found at 1681
    Illegal PstI site found at 1859
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 1923
    Illegal PstI site found at 1681
    Illegal PstI site found at 1859
    Illegal NgoMIV site found at 1357
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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