Composite

Part:BBa_K4806213

Designed by: Luca Langenberg   Group: iGEM23_RPTU-Kaiserslautern   (2023-09-20)
Revision as of 08:46, 21 September 2023 by Lulang (Talk | contribs)


CYP3A4 gene with mNeonGreen for Chlamydomonas reinhardtii (Phytobrick)


This composite part contains the AβSAP(i)-promotor (BBa_K4806013), the coding sequence of the POR (BBa_K4806003), mNeonGreen (BBa_K4806006) and the tRPL23-terminator (BBa_K3002006)*. This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. This level 2 part leads to potential detoxification of specific chemicals (Ohkawa & Inui, 2015).


Construct

Fig.1 Construct design
This construct was designed using the modular cloning system (MoClo).

The resistance cassette for hygromycin is already built in the level 2 vector pMBS810 we are using. The usage of this vector allows the direct assembly of level 0 parts to level 2 constructs, facilitating the cloning time (Niemeyer & Schroda, 2022).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1353
    Illegal PstI site found at 2613
    Illegal PstI site found at 2673
    Illegal PstI site found at 3332
    Illegal PstI site found at 4144
    Illegal PstI site found at 4551
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal NheI site found at 2538
    Illegal PstI site found at 1353
    Illegal PstI site found at 2613
    Illegal PstI site found at 2673
    Illegal PstI site found at 3332
    Illegal PstI site found at 4144
    Illegal PstI site found at 4551
    Illegal NotI site found at 2978
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4160
    Illegal XhoI site found at 530
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1353
    Illegal PstI site found at 2613
    Illegal PstI site found at 2673
    Illegal PstI site found at 3332
    Illegal PstI site found at 4144
    Illegal PstI site found at 4551
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1353
    Illegal PstI site found at 2613
    Illegal PstI site found at 2673
    Illegal PstI site found at 3332
    Illegal PstI site found at 4144
    Illegal PstI site found at 4551
    Illegal NgoMIV site found at 5344
    Illegal AgeI site found at 268
    Illegal AgeI site found at 4489
  • 1000
    COMPATIBLE WITH RFC[1000]


Results

[edit]
Categories
Parameters
None