Translational_Unit

Part:BBa_K4586026

Designed by: Mohamed Mohamed Saad Aboelghare   Group: iGEM23_AFCM-Egypt   (2023-09-15)
Revision as of 14:39, 20 September 2023 by Mohamed-Ab0elghare (Talk | contribs) (→‎Part Description)


Loading system (CD63-L7Ae)


This composite part coding for our loading system that is composed of two components: L7Ae, which is a RNA binding protein that have an affinity to C\D box or kink turn coupled present within the 3` end of our cargo. This RNA binding protein is conjugated to the C terminal end of CD63, which is highly expressed trans membrane protein on exosomes surface.

Usage

This part is implemented in our design to represent the internal domain of the syn notch receptor, which controls the level of our therapeutic agent by releasing the transcription factor VP64, which triggers the expression of the internal circuit that secretes the exosome's cargo, which is regulated by ZF 21-16 promoter activity, which is composed of three main components.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None