Composite

Part:BBa_K4887022:Design

Designed by: Duoqing LIN   Group: iGEM23_Shanghai-BioX   (2023-09-19)
Revision as of 06:31, 27 September 2023 by Htclamm (Talk | contribs)


Expression vector of IbGBSSI knockout system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2106
    Illegal PstI site found at 3528
    Illegal PstI site found at 3732
    Illegal PstI site found at 3762
    Illegal PstI site found at 4974
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2106
    Illegal PstI site found at 3528
    Illegal PstI site found at 3732
    Illegal PstI site found at 3762
    Illegal PstI site found at 4974
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1567
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2106
    Illegal PstI site found at 3528
    Illegal PstI site found at 3732
    Illegal PstI site found at 3762
    Illegal PstI site found at 4974
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2106
    Illegal PstI site found at 3528
    Illegal PstI site found at 3732
    Illegal PstI site found at 3762
    Illegal PstI site found at 4974
    Illegal NgoMIV site found at 1157
    Illegal NgoMIV site found at 1176
    Illegal NgoMIV site found at 2394
    Illegal NgoMIV site found at 3498
    Illegal NgoMIV site found at 3571
    Illegal NgoMIV site found at 4056
    Illegal NgoMIV site found at 4965
    Illegal AgeI site found at 6940
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The vector for CRISPR-knockout expression, which is constructed after the part BBa_K4887021 (backbone of IbGBSSI Knockout system) is inserted into the binary vector pCAMBIA1301,will be transferred into Agrobacterium tumefaciens which is used to infect embryogenic calli of sweet potato.


Source

The part is constructed after the part BBa_K4887021 (framework of IbGBSSI Knockout system) is inserted into the binary vector pCAMBIA1301 .

References