Part:BBa_K4806219

CYP81A10V7 gene with HA-tag for Chlamydomonas reinhardtii (Phytobrick)

This composite part contains the AβSAP(i)-promotor (BBa_K4806013), the coding sequence of CYP81A10V7 (BBa_K4806005), the HA-tag (BBa_K3002017)^* for detection and the tRPL23-terminator (BBa_K3002006)^*. This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. This level 2 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015).

Construct

Fig.1 Construct design
This construct was designed using the modular cloning system (MoClo).

The resistance cassette for spectinomycin is already built in the level 2 vector pMBS807 we are using. The usage of this vector allows the direct assembly of level 0 parts to level 2 constructs, facilitating the cloning time (Niemeyer & Schroda, 2022).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 2427
  Illegal PstI site found at 2569
  Illegal PstI site found at 3191
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 249
  Illegal PstI site found at 2427
  Illegal PstI site found at 2569
  Illegal PstI site found at 3191
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 530
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 2427
  Illegal PstI site found at 2569
  Illegal PstI site found at 3191
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 2427
  Illegal PstI site found at 2569
  Illegal PstI site found at 3191
  Illegal NgoMIV site found at 2274
  Illegal NgoMIV site found at 3636
  Illegal AgeI site found at 268
  
• 1000
  COMPATIBLE WITH RFC[1000]