Regulatory

Part:BBa_K203113:Design

Designed by: Lars Velten, Chenchen Zhu, Michael Bartoschek and Simon Haas   Group: iGEM09_Heidelberg   (2009-10-15)
Revision as of 15:49, 16 October 2009 by Larsplus (Talk | contribs) (→‎Source)

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JeT proximal-CMV core; constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CMV core promoter was amplified from pcDNA5/FRT (invitrogen) by using ATCGAAGCTTGGCGGTAGGCGTGTA as a forward and CAGTGCTAGCatttcgataagccagtaagc as a reverse primer. PCR product and Part:BBa_K203112 were cut with SpeI and HindIII, and ligated.

Source

For the CMV core promoter: pcDNA5/FRT (invitrogen)
For the JeT proximal promoter: Generated from Part:BBa_K203112

References