Part:BBa_K203112
JeT; constitutive promoter
A constitutive reference promoter for gene expression and relative promoter characterization in mammalian cells. Contains a HindIII site between core and proximal promoter for creation of new promoters.
Usage and Biology
Mammalian promoters can be subdivided into several "domains". The core promoter is the binding site of the basal transcription machinery, i.e. RNA polymerase and associated factors. Core promoters differ in composition, but are more or less similar for most genes [1]. The main regulatory domain is the proximal promoter, which is where regulatory elements bind. It can be very large (4kb), meaning that some transcription factors regulate transcription despite being very far away from the RNA polymerase. If the core promoter prevents optimal binding of the basal transcription machinery, promoter strength varies without affecting regulation.
[1] Heintzman ND, Ren B. The gateway to transcription: identifying, characterizing and understanding promoters in the eukaryotic genome. Cellular and Molecular Life Science 64, 386-400 (2007).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
Has by definition of [http://2009.igem.org/Team:Heidelberg/Project_Measurement REU] an expression strength of 1 [http://2009.igem.org/Team:Heidelberg/Project_Measurement REU]. Has by definition of [http://2009.igem.org/Team:Heidelberg/Project_Measurement RMPU] an expression strength of 1 [http://2009.igem.org/Team:Heidelberg/Project_Measurement RMPU].
| None |