![](https://parts.igem.org/images/partbypart/icon_composite.png)
Part:BBa_K4656007
Plam-TPH-TDC
![](https://static.igem.wiki/teams/4656/wiki/part-plam-tph-tdc.jpg)
Usage and Biology
To verify the feasibility of the metabolic module pathway, that is, the pathway for the production of sufficient tryptophan hydroxylase (TPH) and tryptophan decarboxylase (TDC) in engineered bacteria. Using components BBa_R0051, BBa_K4656002 and BBa_K4656006, we designed a gene route Plam-TPH1-TDC1-EGFP, cloned it into the target vector pET28a(+), and then transformed the engineered bacteria. The routes formed by these components can be used to verify the viability of metabolic modules. With untreated pET28a as the control group, we conducted experiments such as WB, fluorescence intensity (OD600) determination, gray value analysis, etc., to verify the feasibility of the metabolic module pathway.
Experimental results
1.Western blotting results:
![](https://static.igem.wiki/teams/4656/wiki/part007.jpg)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 959
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2641
Illegal BamHI site found at 2125
Illegal BamHI site found at 2227 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1733
Illegal NgoMIV site found at 2168
Illegal NgoMIV site found at 2723
Illegal AgeI site found at 734
Illegal AgeI site found at 1871
Illegal AgeI site found at 2795 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 301
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