Part:BBa_K4606007

Vector-AmpR

description

In this part, we designed a plasmid for fusion protein recombination. With this device, we can insert the coding sequence of a novel protein before Poly A. When transfected into E. coli or other recipient cells, it amplifies and expresses the corresponding gene product.

Due to the addition of the AmpR gene to the plasmid, we could easily confirm whether the recombination was successful or not, as only the recombinants can grow into colonies on the medium supplemented with Ampicillin.

Figure 1. Restriction digest verification of vector plasmid by PCR.
1#: DNA marker. 2#: Enzyme digestion product of the vector plasmid. 3#: Control vector plasmid.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]