Part:BBa_K4586004
Cas12k
Part Description
This part codes for the type V-K CRISPR-Cas system effector protein Cas12k, that is characterized by naturally inactivated RuvC domain and associated with Tn7-like transposon for RNA-guided DNA or genome editing, Its protospacer adjacent motif (PAM) sequence is GGTT. that is essential for activating the enzymatic activity of the Cas protein, and the guide RNA is designed based on the PAM sequence site corresponding to the target gene.
Usage
This part is implemented in our design to be the main element of our therapeutic agent (Cargo) to cause apoptosis in the autoreactive B-cells that secrete ACPA. Its guide RNA is designed to drive the protein portion of the CRISPR Cas system Cas12k to the BAFF-R gene, which is essential for B-cell survival and maintenance.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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