Part:BBa_K4768009

Split T7 RNA polymerase (Nterm) conjugated to rapamycin antibody (FRB) with a soluble linker

Part for expression of the Split T7 RNA polymerase (Nterm) conjugated to rapamycin antibody (FRP) with a soluble linker

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 45
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 641
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 45
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 45
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 26
  

Introduction

Figure 1: NT7-TM-FRB structure.

The CALIPSO part BBa_K4768009 is composed of the N-terminal fragment of the T7 RNA polymerase (residues 1 to 180) fused to an anti-rapamycin antibody FRB through a soluble linker (SL). This gene is under transcriptional control of an SP6 promoter and T7 terminator.

This part, coupled to the part

Figure 2: Gel electrophoresis analysis of the PCR products generated during the two-step amplification reactions. 0.8% agarose gel and EtBr staining were used. (A) Amplification products of the first PCR appeared at the expected size. (B) Amplification products of the second PCR appeared also at the expected size.