Coding
Part:BBa_K4768008
Designed by: BOABEKOA Pakindame Group: iGEM23_Toulouse-INSA-UPS (2023-09-14)
Split T7 RNA polymerase (Nterm) conjugated to Pertuzumab with a transmembrane linker
Part for Expression of the T7 RNA polymerase (Nterm) conjugated to Pertuzumab with a transmembrane linker ZipA
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 987
Illegal XbaI site found at 40
Illegal SpeI site found at 1147 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 987
Illegal SpeI site found at 1147 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 987
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 987
Illegal XbaI site found at 40
Illegal SpeI site found at 1147 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 987
Illegal XbaI site found at 40
Illegal SpeI site found at 1147
Illegal AgeI site found at 1200 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 21
Illegal SapI.rc site found at 1836
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Introduction
The CALIPSO part BBa_K4768008 is composed of the N-terminal subunit of the T7 RNA polymerase (residues 1 to 180) fused to the anti-HER2 antibody Pertuzumab through an optimized linker sequence containing a transmembrane domain. This gene is under transcriptional control of an SP6 promoter and T7 terminator.
Construction
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Molecular Modeling
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Characterisation
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Conclusion and Perspectives
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References
- article 1 xxxxxxxx
- article 2 xxxxxxx
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Categories
Parameters
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