Part:BBa_K4656000

PchA

The pchA is present in the butyrate sensor derived from the EHEC strain O157: H7, which was discovered by Nakanishi et al. (2009)[1]. The butyrate sensing system includes sequences of pchA promoters (PpchA) and pchA regulators located upstream, and LEE1 promoters, PLee1 and RBS located downstream. Nakanishi et al. found that leucine response regulatory protein (Lrp) forms a complex with butyrate, which activates the transcription of pchA regulatory factors after binding to the PpchA. The pchA regulator then binds to the PLee1 to transcribe the ler gene. In short, it is a regulatory gene(regulates LEE1 gene, encodes pchA protein).

Usage and Biology

In our butyrate-sensing circuit, pchA is control by PpchA，which can sense changes in butyrate concentration. PchA binds the promoter of LEE1, PLEE1, and then help to activate the expression of its downstream gene. Experimental results about our butyrate-sensing circuit can be found in our composite part BBa K4656008

Reference

[1]. Nakanishi, N., et al., Regulation of virulence by butyrate sensing in enterohaemorrhagic Escherichia coli. Microbiology (Reading), 2009. 155(Pt 2): p. 521-530.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 268
  
• 1000
  COMPATIBLE WITH RFC[1000]