Part:BBa_K4656007:Experience

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Applications of BBa_K4656007

To verify the feasibility of the metabolic module pathway, that is, the pathway for the production of sufficient tryptophan hydroxylase (TPH) and tryptophan decarboxylase (TDC) in engineered bacteria. Using components BBa_K4656001, BBa_K4656002 and BBa_K4656006, we designed a gene route Plam-TPH1-TDC1-EGFP, cloned it into the target vector pET28a(+), and then transformed the engineered bacteria. The routes formed by these components can be used to verify the viability of metabolic modules. With untreated pET28a as the control group, we conducted experiments such as WB, fluorescence intensity (OD600) determination, gray value analysis, etc., to verify the feasibility of the metabolic module pathway.

WB result:

WB result:

gray-value result:

metabolism result:

User Reviews

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