Other
Part:BBa_K4891010:Design
Designed by: Kexin Fei Group: iGEM23_Guangzhou-MedX (2023-06-27)
sgRNAaroK
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
These recombinant plasmids pTargetF-sgRNAaroK/aroL serve as homologous fragments and guide RNA for knocking out the target genes by CRISPR-Cas9, respectively.
Source
AroK and AroL (encoding shikimic acid kinase) are obtained from the genome of E. coli MG1655. In addition, recombinant plasmids pTargetF-sgRNAaroK/aroL initiate with pTargetF, which stores in our lab.