Composite

Part:BBa_K4890011

Designed by: Duoqing LIN   Group: iGEM23_Shanghai-SDG   (2023-08-14)
Revision as of 10:36, 5 October 2023 by Htclamm (Talk | contribs)


UAS-Hsp70

This part contains transcription activator and promoter derived from pUAST plasmid. It consists of UAS sequence (BBa_K3281012) and Hsp70 sequence (BBa_K4890004). Upstream activating sequence (UAS) is a cis-acting regulatory sequence. It increases the expression of a neighbouring gene when binds to GAL4. Hsp70 is a promoter that can bind to RNA polymerase and start transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 226
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 226
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 226
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 226
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None