Primer

Part:BBa_K4790042

Designed by: Yi Zheng   Group: iGEM23_NNU-CHINA   (2023-08-14)
Revision as of 12:11, 4 October 2023 by Nitta (Talk | contribs)

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ASR1-M40E-F

The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on node 59 and coding the 40th amino acid from methionine to glutamic acid. According to Codon preference in E.coli, GAA has the highest possibility coding glutamic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None