Composite

Part:BBa_K4656010:Design

Designed by: Xinji Gu   Group: iGEM23_NMU-China   (2023-08-10)
Revision as of 09:24, 10 August 2023 by GuXinji (Talk | contribs) (→‎Source)

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PRM-CI857-PR-phIF-MazE-PphIF-MazF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1625
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In summer, the temperature in some areas can reach more than 37℃, resulting in engineered bacteria in these places can survive even if they leave the human body. So there is still a risk of damaging the environment after being discharged from the human body. This point needs to be considered carefully.


Source

The PR, PRM are derived from Lambda phage.

The T7 terminator is derived from E. coli (+LVA).

The MazF and MazE are derived from Bacillus subtilis.

The phIF and PphIF are derived from Pseudomonas protegens Pf-5.

References