Coding

Part:BBa_K4765013

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-08-07)
Revision as of 07:52, 11 August 2023 by Siliang Zhan (Talk | contribs)


Nb3, R4P43 from 10.1016/j.cell.2018.06.041

Introduction

MAA analogues like shinorine or porphyra-334 is transformed from MG by a D-Ala-D-Ala ligaselike enzyme gene MysD[1].

Usage and Biology

We performed codon optimization on BBa_K4273013 (NlmysD) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysD catalyzes the fourth reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 166


References

  1. ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.
[edit]
Categories
//cds/enzyme
Parameters
protein