Coding

Part:BBa_K4656000:Design

Designed by: Xinji Gu   Group: iGEM23_NMU-China   (2023-08-07)
Revision as of 02:47, 9 August 2023 by GuXinji (Talk | contribs)


PchA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To add a fluorescent output or any other type of output protein, simply add the base pairs downstream of RBS.


Source

The pchA is from its organism, the enterohemorrhagic Escherichia coli strain O157:H7.

References

[1]. Nakanishi, N., et al., Regulation of virulence by butyrate sensing in enterohaemorrhagic Escherichia coli. Microbiology (Reading), 2009. 155(Pt 2): p. 521-530.