Plasmid
Part:BBa_K4606000
Designed by: Baochao Li Group: iGEM23_Nanjing-NFLS (2023-08-04)
Vector-AmpR
MiR-3074-5p is a kind of microRNA, which can participate in the physiological and pathological activities of cells by regulating the expression of target genes. And in our system, we found that miR-3074-5p targeted SMAD4 gene in chondrocytes and disrupt the homeostasis of chondrocytes.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1163
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 12
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1163
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1163
Illegal NgoMIV site found at 487 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 279
Illegal BsaI.rc site found at 2637
Illegal SapI site found at 1557
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Categories
Parameters
None |