Coding
Part:BBa_K4891014
Designed by: Kexin Fei Group: iGEM23_Guangzhou-MedX (2023-06-27)
glk-glf
It is the key part that is responsible for strengthening glucose utilization rate. The phosphotransferase system was replaced by heterogeneously introducing glucose facilitator in strain E. coli ptsG::glk-glf.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 124
Illegal PstI site found at 1136
Illegal PstI site found at 1327
Illegal PstI site found at 1900 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 124
Illegal PstI site found at 1136
Illegal PstI site found at 1327
Illegal PstI site found at 1900 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 124
Illegal PstI site found at 1136
Illegal PstI site found at 1327
Illegal PstI site found at 1900 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 124
Illegal PstI site found at 1136
Illegal PstI site found at 1327
Illegal PstI site found at 1900
Illegal AgeI site found at 421
Illegal AgeI site found at 1312
Illegal AgeI site found at 1468
Illegal AgeI site found at 1480
Illegal AgeI site found at 2191 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |