Coding
Part:BBa_K4891004:Design
Designed by: Kexin Fei Group: iGEM23_Guangzhou-MedX (2023-06-27)
glf
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 155
Illegal PstI site found at 346
Illegal PstI site found at 919 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 155
Illegal PstI site found at 346
Illegal PstI site found at 919 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 155
Illegal PstI site found at 346
Illegal PstI site found at 919 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 155
Illegal PstI site found at 346
Illegal PstI site found at 919
Illegal AgeI site found at 331
Illegal AgeI site found at 487
Illegal AgeI site found at 499
Illegal AgeI site found at 1210 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Glucose facilitator serves as an alternative for enhancing glucose transportation rate.
Source
Glucokinase encoded by glf gene from Zymomonas mobilis is codon-optimized and synthesized