Plasmid_Backbone

Part:BBa_K187000:Experience

Designed by: Team BioBytes, University of Alberta   Group: iGEM09_Alberta   (2009-10-15)
Revision as of 04:23, 16 October 2009 by JuliaPon (Talk | contribs) (→‎Applications of BBa_K187000)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K187000

This part has been tested extensively by the BioBytes team, and has worked as expected in all assays. See the Alberta wiki for more information: http://2009.igem.org/Team:Alberta. The plasmids pBA and pAB have been used in all assays for the bead assembly method, as described in the BioBytes BBF RFC.

Briefly, Biobytes is a new standardized in vitro assembly method. The system is based on a bead platform where constructs with long sticky ends are annealed together sequentially. This system is designed to enable the single day assembly of plasmids composed of up to about half a dozen premade parts. Complementary to existing BioBrick standards for DNA assembly, we expect this system to be useful for rapid generation of large constructs wherever an interpart spacing of 37 bp can be tolerated.

User Reviews

UNIQ7511742468131e4b-partinfo-00000000-QINU UNIQ7511742468131e4b-partinfo-00000001-QINU