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Part:BBa_K4260031

Designed by: Claudia Angélica García Alonso   Group: iGEM22_TecCEM   (2022-10-13)
Revision as of 02:26, 14 October 2022 by JAMUNOZ (Talk | contribs)


Promoter, for Iem & Iso

This is a bidirectional promoter region that helps the transcription of two different genes, one in the leader chain of DNA and other in the antisense chain. This type of arrangement is commonly found in operons, in bacteria. This part promotes the transcription of IemR, a trancriptional activator, and together both parts promote the transcription of the coding gene of isoeugenol monooxygenase. 

         <img
             src="https://parts.igem.org/File:Promotor_pseudomonas.jpeg"
             alt="Fig1-sp"
             width="700"
             align="center"
           />


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 164


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