Composite

Part:BBa_K200024

Designed by: Royah Vaezi   Group: iGEM09_Imperial College London   (2009-10-15)
Revision as of 16:03, 18 October 2009 by JamesField (Talk | contribs)

RcsB+TT

Colanic acid producing gene with double terminator

RcsB (BBa_K200000) coupled to the double terminator (BBa_B0015).


RcsB overexpression turn on the RCS phosphorelay system. This results in the modulation of ~5% of the E.coli genome. One of the most notable effects of this is the production of the exopolysaccharide colanic acid.

The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.

The double terminator allows the RcsB gene to be incorporated into a closed transcriptional unit.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None