Composite

Part:BBa_K4444017:Design

Designed by: Cristina Perez   Group: iGEM22_UPenn   (2022-10-12)
Revision as of 05:29, 13 October 2022 by Ciperez (Talk | contribs) (→‎Design Notes)

Design Notes

Modifications to FRB-nTEV:

- FRB is replaced with iLID

- ER localization tag is added at beginning

- mCherry is added between iLID and nTEV


Components:

ER localization tag, iLID, mCherry, nTEV

ilidntev.png

Source

FKBP-nTEV Backbone: https://www.addgene.org/179675/

mCherry: https://www.addgene.org/164037/

iLID: Lab member’s plasmid (William Benman) – pHR sspB(nano)-BFP-SOS-2A-iLID

References

[1] Wehr, M., Laage, R., Bolz, U. et al. Monitoring regulated protein-protein interactions using split TEV. Nat Methods 3, 985–993 (2006). https://doi.org/10.1038/nmeth967

[2] Vlahos, Alexander E., et al. “Protease-Controlled Secretion and Display of Intercellular Signals.” Nature Communications, vol. 13, no. 1, 17 Feb. 2022, p. 912, www.nature.com/articles/s41467-022-28623-y, 10.1038/s41467-022-28623-y.