Coding

Part:BBa_K4307011

Designed by: Chi Zhang   Group: iGEM22_Tsinghua   (2022-09-30)
Revision as of 15:49, 12 October 2022 by YuheWang (Talk | contribs)


NisB

Originated from Lactococcus lactis subsp. Lactis, NisB is involved in the post-translational modification of the lantibiotic nisin. We managed to express NisB in E.coli for post-translational modification of NisA-affi fusion protein.
Functions together with NisA-affi(BBa_K4307010) and NisC(BBa_K4307012), it forms composite part NisA-affi-NisB+NisC (BBa_K4307014) to produce functional NisA-affibody peptide.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 679
    Illegal PstI site found at 2845
    Illegal PstI site found at 2944
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 679
    Illegal PstI site found at 2845
    Illegal PstI site found at 2944
    Illegal NotI site found at 1385
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 687
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 679
    Illegal PstI site found at 2845
    Illegal PstI site found at 2944
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 679
    Illegal PstI site found at 2845
    Illegal PstI site found at 2944
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

The following figure demonstrates our successful construction.


bba-k4307011-gel.png

               Figure 1:  The construction results of NisB.





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