Regulatory

Part:BBa_K4158013:Experience

Designed by: Nanami Onishi, Eri Ito   Group: iGEM22_Waseda_Tokyo   (2022-09-25)
Revision as of 15:51, 12 October 2022 by N Onishi (Talk | contribs) (→‎Applications of BBa_K4158013)

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in vitro experiments were conducted. tube3 shows fluorescence before incubation without ITPG. On the other hand, in tube3-1 at 150 min after the addition of IPTG, the activity of gfp was decreased. Therefore, it is thought that the activity of gfp is regulated by the addition of IPTG. When IPTG is added, EdcR is purified by PET-T7-edcR. This result suggests that the suppression of gfp is caused by the generation of EdcR.

Fig. 1. FITC values before incubation (left) and after 150min when FITC is 5M

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