Composite

Part:BBa_K4477013:Design

Designed by: Miranda Khoury   Group: iGEM22_Virginia   (2022-09-06)
Revision as of 13:08, 12 October 2022 by Mirandakhoury (Talk | contribs) (→‎Source)


McPC603 anti-oxLDL scFv - complete expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 446
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 752
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CDS was codon optimized for expression in SHuffle bacteria, removal of illegal restriction sites.


Source

1. https://www.sciencedirect.com/science/article/pii/S0769262585800581
2. https://pubmed.ncbi.nlm.nih.gov/14644097/

References

3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312285/
4. https://www.pnas.org/doi/epdf/10.1073/pnas.71.11.4298