Part:BBa_K4129121

The fungal synthetic expression system with FunsTF02 and 6xLexO-Pmin

Fungal synthetic expression system using FunsTF02

In this fungal synthetic expression system (SES) FunsTF02 was constitutively expressed from PgpdA. The FunsTF02, which contains a LexA DNA-binding domain, would be able to bind to the LexO binding sites for the minimal promoter fused with LexO binding sites (6xLexO-Pmin). The efficiency of the FunsTF05 activation was measured by the reporter gene mCherry, which is expressed from the 6xLexO-Pmin.

For characterisation look up BBa_K4129101.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 4123
  Illegal XbaI site found at 6036
  Illegal PstI site found at 141
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 911
  Illegal NheI site found at 2935
  Illegal PstI site found at 141
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 145
  Illegal BamHI site found at 3461
  Illegal BamHI site found at 5317
  Illegal XhoI site found at 701
  Illegal XhoI site found at 1083
  Illegal XhoI site found at 3610
  Illegal XhoI site found at 3987
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 4123
  Illegal XbaI site found at 6036
  Illegal PstI site found at 141
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 4123
  Illegal XbaI site found at 6036
  Illegal PstI site found at 141
  Illegal NgoMIV site found at 3027
  Illegal NgoMIV site found at 5953
  Illegal AgeI site found at 1290
  Illegal AgeI site found at 1438
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1117
  Illegal BsaI.rc site found at 5667
  Illegal SapI site found at 323