Composite

Part:BBa_K174014

Designed by: The Newcastle 2009 iGEM team   Group: iGEM09_Newcastle   (2009-10-14)
Revision as of 13:04, 14 October 2009 by Goksel (Talk | contribs)

cwlJ + sleB, B. subtilis germination genes

Both CwlJ and SleB are germination specific cortex hydrolases. Although SleB has a major effect for the germination, cwlJ's effect is minor. However Bacillus subtilis with double mutation without cwlJ and sleB were not able germinate again (2).

To control the germination rate tightly, cwlJ and sleB genes are combined to make this device.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None