Part:BBa_K243000
Protein domain (active) of the restriction endonuclease FokI
This part is used as the active domain of our universal restriction endonuclease. It cut DNA, when it fused with the inactive protein domain of our universal restriction endonuclease(BBa_K243001)and linked with specific oligos.
Sequence specific nuclease(1968)
The researchers H.O. Smith, K.W. Wilcox, and T.J. Kelley (Johns Hopkins University 1968), were the first persons who isolated and characterized the first restriction nuclease whose functioning depended on a specific DNA nucleotide sequence. This was a big breakthrough for the genetic engineering, it gave the scientists a tool for working with the DNA. Now over forty years later over 3000 restriction enzymes have been studied in detail, and more than 600 of these are available commercially and are routinely used for DNA modification and manipulation in laboratories.
Usage and Biology
The usage of an universal endonuclease could change the daily routine of a scientist, who is working with DNA, because the question where to cut with which enzyme isn't needed anymore. He is free to chose the cutting sequence and can received the part that he wanted. The only thing is he had to plan where to cut and ordered specific oligos.
The idea
The idea is based of the modification of the existing restriction endonuclease FokI. Through modifications in the amino-acids sequence the DNA binding domain was diluted and one cleavage domain was inactivated
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 487
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