Composite

Part:BBa_K4165175

Designed by: Ahmed Sameh   Group: iGEM22_CU_Egypt   (2022-09-30)
Revision as of 23:35, 13 October 2022 by SalmaMohsen 2000 (Talk | contribs) (→‎WetLab Results)

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Biobrick His - Tau

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), RBS 4 (BBa_K4165262), 6x His-tag (BBa_K4165020), Tau (BBa_K4165009), and T7 terminator (BBa_K731721).

Usage and Biology

In the AD brain, tau Hyperphosphorylation is considered the main cause of AD progression, it may alter the protein's shape and charge, which in turn causes the microtubule-binding domain to become exposed and allow tau to self-assemble and form oligomers characterized to be a neurofibrillary tangle. According to several studies, the polymerized tau (neurofibrillary tangles) is inert since it does not bind to tubulin or encourage its assembly into microtubules, the His Tag for the purification of the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 169
    Illegal AgeI site found at 355
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 333
    Illegal BsaI site found at 1245
    Illegal BsaI.rc site found at 45
    Illegal SapI.rc site found at 519

Mathematical Modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab. 


                 Figure 1. this figure shows the results from the transcription and translation code showing 
                the variation of mRNA and protein concentrations with time compared with the wet lab results.


WetLab Results

Comparison between the mathematical model of dry lab and the wet lab results of His Tau using chemical lysis

We have made a statistical analysis (t-test) to investigate the mathematical model's effectiveness in predicting the amount of the produced protein due to IPTG induction, whereas the test compares the mathematical model expected results to the replicates made in the induction experiment, given that all the condition of the experiment has been input to the model generating the predicted results 

           Figure 2. This graph shows the correlation between the mathematical model of the dry lab and the wet lab 
                                           results of His Tau using chemical lysis.


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