DNA

Part:BBa_K4438704

Designed by: Shreyas Samir Parkhie   Group: iGEM22_IISER-Tirupati_India   (2022-10-12)
Revision as of 11:58, 12 October 2022 by Shreyasparkhie17 (Talk | contribs)

P4G13_Target_2

P4G13_Target_1 (BBa_K4438702) is a single-stranded DNA sequence having 84 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 3’ end has few bases complementary to P4G13_trigger_2_w/oPol (BBa_K4438703). In the middle complete ssDNA antisense T7 promoter sequence is present. The 5’ end has broccoli cDNA sequence.

Usage and Biology

This part along with P4G13_trigger_2_w/oPol (BBa_K4438703) and P4G13_aptamer (BBa_K4438700) can be used for sensing different concentrations of progesterone [1].



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Contreras Jiménez, G., Eissa, S., Ng, A., Alhadrami, H., Zourob, M., & Siaj, M. (2015). Aptamer-based label-free impedimetric biosensor for detection of progesterone. Analytical chemistry, 87(2), 1075-1082.

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