Composite

Part:BBa_K4204021:Design

Designed by: Jianfei Song   Group: iGEM22_BNDS_China   (2022-10-12)
Revision as of 11:45, 12 October 2022 by Jianfei Song (Talk | contribs) (→‎Source)


IPTG induced LysPBC5 with ProQC system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 350
    Illegal NheI site found at 1468
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1045
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1097
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The toehold switch would be at the 5' end of the mRNA with a corresponding trigger at the 3' end of the mRNA. This synthetic protein quality control system is believed to have no limitations regarding its use in other bacteria, and this system can be applied to improve the quality of recombinant protein production and also efficiencies of metabolic pathways.

Source

The protein quality control system is from Yang et al's work [1], the CDS of LysPBC5 is from Lee et al's work [2], and other components are from BBa_K653001. Assembled by BNDS_China 2022.

References

[1] Yang J, Han YH, Im J, Seo SW. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol. 2021 Apr;17(4):421-427. doi: 10.1038/s41589-021-00736-3. Epub 2021 Feb 4. PMID: 33542534.

[2] Lee, K. O., Kong, M., Kim, I., Bai, J., Cha, S., Kim, B., Ryu, K.-S., Ryu, S., & Suh, J.-Y. (2019). Structural basis for cell-wall recognition by bacteriophage PBC5 endolysin. Structure, 27(9). https://doi.org/10.1016/j.str.2019.07.001