gshF

Description

gshF encodes a bifunctional glutathione synthetase GshF originated from Streptococcus thermophilus.

Usage

It is less sensitive to GSH, is and codon optimized for expressing in G. hansenii.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 178
Illegal EcoRI site found at 760
Illegal EcoRI site found at 1195
Illegal EcoRI site found at 1693
Illegal PstI site found at 19
Illegal PstI site found at 49
Illegal PstI site found at 406
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 178
Illegal EcoRI site found at 760
Illegal EcoRI site found at 1195
Illegal EcoRI site found at 1693
Illegal PstI site found at 19
Illegal PstI site found at 49
Illegal PstI site found at 406
Illegal NotI site found at 1838
Illegal NotI site found at 2038
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 178
Illegal EcoRI site found at 760
Illegal EcoRI site found at 1195
Illegal EcoRI site found at 1693
Illegal XhoI site found at 2019
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 178
Illegal EcoRI site found at 760
Illegal EcoRI site found at 1195
Illegal EcoRI site found at 1693
Illegal PstI site found at 19
Illegal PstI site found at 49
Illegal PstI site found at 406
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 178
Illegal EcoRI site found at 760
Illegal EcoRI site found at 1195
Illegal EcoRI site found at 1693
Illegal PstI site found at 19
Illegal PstI site found at 49
Illegal PstI site found at 406
Illegal NgoMIV site found at 1620
Illegal NgoMIV site found at 2251
1000
COMPATIBLE WITH RFC[1000]

2022 SZPT-China

Characterization

1. Verification of Western blot electrophoresis in E. coli.

Figure1: Verification of Western blot electrophoresis in E. coli.

As shown in Figure 1, the expression of GshF were verified successfully by Western blot.

2.References

[1] Li, W., Li, Z., Yang, J. & Ye, Q. Production of glutathione using a bifunctional enzyme encoded by gshF from Streptococcus thermophilus expressed in Escherichia coli. J. Biotechnol. 154, 261-268 (2011).

[2] Pophaly, S. D. et al. Glutathione biosynthesis and activity of dependent enzymes in food-grade lactic acid bacteria harbouring multidomain bifunctional fusion gene (gshF). J. Appl. Microbiol. 123, 194-203 (2017).