RNA

Part:BBa_K4501002:Experience

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-08)
Revision as of 10:53, 12 October 2022 by Paumarin99 (Talk | contribs)

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This experience page is provided so that any user may enter their experience using this part.
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Applications of BBa_K4501002

We demonstrated the downregulation of myc by transiently transfecting (by electroporation) these shRNA in a expression cassette on Ramos and HEK293T cell lines. qPCR from total RNA extraction shows as high as almost 2-fold decrease in myc expression when treatment with shRNA.

ShRNA qPCR.jpg Figure 1. Results of qPCR for Ramos and HEK293T cell line.

User Reviews

UNIQ0db467d7b436d962-partinfo-00000000-QINU UNIQ0db467d7b436d962-partinfo-00000001-QINU