Composite

Part:BBa_K4121051

Designed by: Ziyu Li   Group: iGEM22_NEFU_China   (2022-10-05)
Revision as of 09:30, 12 October 2022 by L7 (Talk | contribs)


Expression cassette of TmCrtE with constitutive promoter

Expression cassette of TmCrtE with constitutive promoter

The composite part is called "pCCW12-TmCrtE-tTDH1". We use pCCW12 as the promoter, tTDH1 as the terminator and the CDS of this part is TmCrtE(from Taxus media). The three basic parts above make up a new transcription unit, it has the fuction to catalyze the conversion of farnesyl pyrophosphate (FPP) to heterologous geranylgeranyl diphosphate(GGPP).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 240
    Illegal XbaI site found at 727
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 240
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 240
    Illegal BglII site found at 589
    Illegal BglII site found at 730
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 240
    Illegal XbaI site found at 727
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 240
    Illegal XbaI site found at 727
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None