Coding

Part:BBa_K4339001

Designed by: Chloe Singleton   Group: iGEM22_Exeter   (2022-10-08)
Revision as of 08:47, 12 October 2022 by Matthewludwig (Talk | contribs) (→‎References)


Alanine tRNA synthetase

Alanine tRNA synthetase is an enzyme that catalyses the binding of alanine to tRNA. Modelling demonstrated that the expression of spider silk proteins (spidroins), which have a number of repetitive alanine regions, would be increased with the co-expression of alanine tRNA synthetase.


Usage and Biology

Sequence and Features

The sequence originates from E. coli MG1655 K-12 (Puteny et al. 1981)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2066
    Illegal PstI site found at 2582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2066
    Illegal PstI site found at 2582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 629
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2066
    Illegal PstI site found at 2582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2066
    Illegal PstI site found at 2582
    Illegal AgeI site found at 709
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1090
    Illegal SapI.rc site found at 1138
    Illegal SapI.rc site found at 1288

References

Putney S et al. Purification and properties of alanine tRNA synthetase from Escherichia coli. A tetramer of identical subunits. Journal of Biological Chemistry. 1981;256(1): 198–204. doi: https://doi.org/10.1016/s0021-9258(19)70119-7

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