Coding

Part:BBa_K283047:Experience

Designed by: Shi Lei   Group: iGEM09_HKU-HKBU   (2009-10-13)
Revision as of 11:05, 14 September 2015 by Triplet (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K283047

User Reviews

UNIQd69bc85218af29ed-partinfo-00000000-QINU UNIQd69bc85218af29ed-partinfo-00000001-QINU

Group: FAFU-CHINA Author: Ruicheng Dai & Changlong Lu Summary: The inductive effects of IPTG in different concentration and time lengths

IPTG inductive effect

We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.


The inductive effect of different concentration IPTG

We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours FAFU-CHINA PART (1).png

The inductive effect of different time length

We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration. FAFU-CHINA PART (2).png