Coding

Part:BBa_K4477003:Design

Designed by: Miranda Khoury   Group: iGEM22_Virginia   (2022-09-06)
Revision as of 07:54, 12 October 2022 by Mirandakhoury (Talk | contribs) (→‎Design Notes)


IK17 (anti-oxLDL) scFv antibody fragment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 486
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid sequence reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle).

Source

Amino acid sequences for IK17 light and heavy chains acquired from NCBI: heavy: https://www.ncbi.nlm.nih.gov/protein/AAK93956.1 light: https://www.ncbi.nlm.nih.gov/protein/AAK93957.1

Literature and other relevant citations: https://www.jacc.org/doi/full/10.1016/j.jacc.2011.07.017 https://web.expasy.org/abcd/ABCD_AH862

References

1. https://www.jacc.org/doi/full/10.1016/j.jacc.2011.07.017
2. https://pubmed.ncbi.nlm.nih.gov/14644097/