Coding

Part:BBa_K4477002:Design

Designed by: Miranda Khoury   Group: iGEM22_Virginia   (2022-09-06)
Revision as of 07:51, 12 October 2022 by Mirandakhoury (Talk | contribs) (→‎Design Notes)


Human/mouse anti-apoB(MDA) (anti-oxLDL) IgG antibody - heavy chain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 73
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1158
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid sequence from IMGT was reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle). Illegal restriction sites were removed via codon optimization.

The variable heavy and constant 1 domains of this heavy chain are human in origin, while the constant 2 and 3 domains are mouse in origin. See Team Virginia 2022's Benchling folder here: https://benchling.com/mrkhoury/f_/YR4zZSUb-virginia-igem-2022-dna-constructs/ for detailed annotations.

Source

Orticumab heavy chain amino acid sequence acquired from IMGT: https://www.imgt.org/3Dstructure-DB/cgi/details.cgi?pdbcode=9635

References